Intrastrand Self-complementary Sequences in Bacillus subtilis DNA

Transforming Activity in Both Complementary Strands of Bacillus subtilis DNA.

Under conditions favoring single-strand transformation, the two complementary strands of Bacillus subtilis DNA, separated by differential complexing with polyriboguanylic acid, have identical transforming activity. Moreover separated single strands, upon renaturation with unmarked (recipient) DNA, form heteroduplex molecules with similar double-strand transformin activity. These findings bear u...

Determination of DNA sequences containing methylcytosine in Bacillus subtilis Marburg.

The methylcytosine-containing sequences in the DNA of Bacillus subtilis 168 Marburg (restriction-modification type BsuM) were determined by three different methods: (i) examination of in vivo-methylated DNA by restriction enzyme digestion and, whenever possible, analysis for methylcytosine at the 5' end; (ii) methylation in vitro of unmethylated DNA with B. subtilis DNA methyltransferase and de...

Transformation of Bacillus subtilis using hybrid DNA molecules constructed by annealing resolved complementary strands.

HE question of whether one or both strands of the transforming deoxyriboTnucleic acid (DNA) molecule is inserted into the host chromosome of Bacillus subtilis has been the subject of several recent reports. BODMER and GANESAN (1964) presented physical evidence for the incorporation of one strand. AYAD and BARKER (1969) in contrast, presented physical evidence suggesting that both strands of the...

Dna-membrane Complexes of Bacillus Subtilis

Stability of reiterated sequences in the Bacillus subtilis chromosome.

The instability of reiterated sequences in the Bacillus subtilis chromosome that was previously reported (M. Young, J. Gen. Microbiol. 130:1613-1621, 1984) results from the presence of a truncated pC194 replication origin together with an intact replication protein A gene in the amplified DNA. Removal of the truncated pC194 replication origin or inactivation of replication protein A stabilizes ...